Interaction Specificities of Some Small Molecules And Proteins With Dna.
Interaction Specificities of Some Small Molecules And Proteins With Dna.
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, the minimum number of base-pairs per binding site). This criterion, 100% binding of I to DNA at saturation, is not obtained by either the CD or the viscometric titration studies described in this work.
Nevertheless, viscometric titration studies of DNA inter- calating sites by cations I (using the same DNA concentration as the CD studies) show almost identical base-pair/cation ratios at saturation as the CD results cited above (see Tabic 4). It is, therefore, concluded (with a high degree of ...certainty) that the induced CD observed for the DNA-- _l-_9 complexes arises from an intercalation mode of binding.
The results of the pmr and Tm studies (cited earlier), and the viscosity studies (below), are consistent with this interpretation .
The induced circular dichroism .in the absorption band of the cations l_-9 upon binding to salmon sperm DNA is shown in Figures 23, 24, and 25. The spectra were obtained under conditions of saturation of the intercalating sites (i.e., further addition of cations I to the DNA solution do not change the observed spectra).
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To quickly assess the difficulty of the text, read a short excerpt:
, the minimum number of base-pairs per binding site). This criterion, 100% binding of I to DNA at saturation, is not obtained by either the CD or the viscometric titration studies described in this work.
Nevertheless, viscometric titration studies of DNA inter- calating sites by cations I (using the same DNA concentration as the CD studies) show almost identical base-pair/cation ratios at saturation as the CD results cited above (see Tabic 4). It is, therefore, concluded (with a high degree of ...certainty) that the induced CD observed for the DNA-- _l-_9 complexes arises from an intercalation mode of binding.
The results of the pmr and Tm studies (cited earlier), and the viscosity studies (below), are consistent with this interpretation .
The induced circular dichroism .in the absorption band of the cations l_-9 upon binding to salmon sperm DNA is shown in Figures 23, 24, and 25. The spectra were obtained under conditions of saturation of the intercalating sites (i.e., further addition of cations I to the DNA solution do not change the observed spectra).
What to read after Interaction Specificities of Some Small Molecules And Proteins With Dna.?
You can find similar books in the "Read Also" column, or choose other free books by Scofield, Rolfe Eaton, 1946- to read onlineMoreLess
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