Intracellular Enzymes : a Course of Lectures Given in the Physiological Laboratory, University of London

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per hour during the next four hours, and at -0024 gm. per hour during the next fifteen hours.
Palladin suggested that this CO2 was formed by a hypothetical " carbonase " enzyme. On replacing the hydrogen by an air current., a fresh outflow of CO2 began, and continued at the rate of -007 to -005 gm. per hour for the next forty hours or more. Its formation was supposed by Palladin'to be due to an oxidase enzyme. The amounts of CO2 evolved from germinating wheat and etiolated vetch leaves varied b
...etween the following limits. They are calculated for 100 gm. of plant substance : — Plant.
In Hydrogen Current (Carbonase).
In Air Current (Oxidase).
Germinating Wheat ....
Etiolated Leaves of Vetch .
1-025 to 1-282 gm.
•100 to -185 gm.
■142 to -245 gm.
No direct observations upon the oxygen intake were made in these particular experiments, but on keeping other (previously frozen) etiolated vetch leaves in a closed volume of air over mercury, and analysing samples from time to time, it was found that the oxygen was absorbed in rough proportion 1 Palladin, Zeit.


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