Laboratory Directions for Beginners in Bacteriology

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Make a hanging drop preparation from a colony from an agar plate, and one from a colony from a gelatin plate, and examine them microscopically. Describe the appearance of the bacteria in each.
Make a cover-glass preparation from the same colonies and stain each with carbol fuchsin. Examine each carefully and make a drawing of a few of the isolated bacteria. Describe (30) the appearance of the bacteria in these preparations.
Inoculate a tube of bouillon, one of agar and one of gela- tin from a w
...ell isolated colony from one of the agar plates.
42. Suggestions for the examination of the plate and roll cultures. Observe the general appearance of the 33 plates, note whether the colonies are well isolated or run together (confluent) ; describe the appearance of the indi- vidual colonies, (a) those on the surface, (b) those in the depth of the medium. Indicate their shape (round, lenticu- lar, flat, convex or spherical). Are the edges sharply defined ? Is the margin even or irregular ? Give their size (diameter in millimeters), and indicate their color (deter- mine shade from a color chart), and consistence.


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