Laboratory Directions for Beginners in Bacteriology; An Introduction to Practical Bacteriology for Students And Practitioners of Comparative And of Human Medicine

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Stain with alkaline methylene-blue and with carbol fuchsin. The preparation should be stained for fully 5 minutes with the alkaline methylene-blue. Also stain a preparation after Gram's method (38).
Examine carefully a fresh culture made directly from a diph- theritic throat, including stained cover-glass preparations.^ Stain with alkaline methylene-blue and by Neisser'smethod. (It is not always possible to obtain these cultures at this particular time, in which case the examination will be pos
...tponed until they are available.) 146. Neisser's Method of Staining Diphtheria Bacteria Neisser has recently recommended the following method of staining, in which 2 solutions are employed, viz : — (a) One gram of methylene-blue (Griibler's) is dissolved in 20 c.c. of 96% alcohol, which is then mixed with 950 c.c. of distilled water and 50 c.c. of glacial acetic acid.
{b) Two grams of Vesuvin are dissolved in i litre of boiling distilled water and filtered.
The cover-glass preparations are stained in {a) for i to 3 seconds, washed in water, and then stained in (3) for from 3 to 5 seconds, again washed in water, dried, and mounted.


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