Laboratory Directions in Histology; a Guide for Use in a Laboratory Course in Histology

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In some cases, however, its disregard affects the results seriously and as a general rule there is a minimum and a maximum time and between them an optimum time that should be adhered to.
(c) After the tissue has been in the fixing fluid a proper length of tinie, it is necessary that it be washed thoroughly to remove the fixer from it. Usually this should be done by means of water or alcohol or both. In general, fixers containing salts insoluble in alcohol or but slightly soluble, as osmic acid
..., chromic acid, potassium dichromate, etc., should be thoroughly washed in water. Fixers, containing picric acid or alcohol should always be removed by alcohol; mercuric chlorid may be washed out by either water or alcohol.
Inadequate washing out of the fixer may either seriously affect the cutting quality of the tissue (if it is to be subsequently im- bedded), the ease with which it can be stained, or there may be formed precipitates in the tissue giving illusory effects, distortions,, or at least a dirty appearance to the preparation.


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