Laboratory Guide in Experimental Pharmacology Directions for the Course Given I
Laboratory Guide in Experimental Pharmacology Directions for the Course Given I
C W Charles Wallis Edmunds
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On, the opposite side expose the carotid artery and in like manner tie a cannula in it (page 20). Fill the venous cannula with physiological salt solution and the carotid cannula with a solution made" by mixing equal volumes of water and a saturated solution of sodium sulphate. 148 LABORATORY GUIDE IN The ordinary form of mercury manometer, which is so well known as to need no description, is used. Fill the proximal limb of the U-shaped tube, i. E. , the limb which is to be con- nected with the... blood vessel, with the sodium sulphate solu- tion, using for this purpose a pipette with a long tapering point. The lead (or rubber) tube, which is connected to the side tube of the proximal limb of the manometer, is to be completely filled with the sulphate solution, taking great care to see that all air is expelled from the tube and manom- eter. Close the clamp on the tubing which connects the lead tube and manometer. With a short piece of rubber tub- ing connect a large pipette (25 cc. ) filled with the sulphate solution to the upper opening of the proximal limb of the manometer and blow in the pipette so as to raise the pres- sure until there is a difference of 10 or 12 cm.
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