Laboratory Guide in Soil Bacteriology

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NaOl.
0.4 gm. Fe^CSOJ^.
0.3 gm. MgSOj.
2. Stir until all the materials are in solution.
3. Sterilize in the autoclave as usual.
74 LABOKATOEY BACTERIOLOGY.
XI. "Nitrate-Nitrogen Transformation" Solution.
1. Dissolve in 1 liter (1,000 ce.) of distilled water : 0.2 gm. K.HPO,.
0.2 gm. MgSO..
0.5 gm. NaNOg.
0.02 gm. CaCl,.
1.0 gm. dextrose.
1 drop. sol. FezClc.
2. Stir until dissolved.
3. Add a few drops of phenolphthalein and then 10% JSTaOH until the solution is Just neutral.
4. Sterilize in the
... autoclave at 15 lbs. pressure for ten minutes.
XII. " Ammonior-Nitrogen Transformation" Solution.
1. Dissolve in 1 liter of distilled water : 0.2 gm. K^HPO..
0.2 gm. MgSO,.
1.0 gm. (NH,)^ SO..
0.02 gm. CaClj.
1.0 gm. dextrose.
1 drop sol. Fe^Clo.
2. Stir until dissolved.
3. Add 10% ISTaOH until the solution is neutral to phe- nolphthalein.
4. Sterilize in the autoclave as usual.
PEEPABATION OF MEDIA. 73 XIII. "'Synthetic" Solution.
1. To 1,000 ec. of tap water add the following : 10 gms. dextrose.


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