Laboratory Manual for the Detection of Poisons And Powerful Drugs

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But neither potassium nitrate nor nitric acid can be used, as these reagents even pre- vent this test. Consequently strychnine nitrate does not give the test.
2. Physiological Test. Dissolve the ether residue in a little very dilute hydrochloric acid. Evaporate the filtered solution to dryness upon the water-bath. Dissolve the residue in pure water (about i cc. ) and inject this solution into the lymph-sac on the back of a lively frog. Keep the experimental frog in a large, loosely covered beak
...er. Toxic symptoms will appear in 5 to 30 minutes, depending upon the quantity of strychnine.
According to Tafel (Annalen der Chemie und Pharmazie, 268, 233 (1892), this color reaction is characteristic of many anilides and is due to the presence of the group -CO-N =.
2 See page 321 for the preparation of this reagent.
100 DETECTION OF POISONS Strychnine does not increase reflex irritability for all kinds of stimuli but only for tactile, optical and especially for acoustic stimuli. When the dose of strychnine is sufficiently large, each kind of stimulus mentioned will produce convulsions like those caused by tetanus.


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