Laboratory Manual of Biological Chemistry With Supplement
Laboratory Manual of Biological Chemistry With Supplement
Otto Folin
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To some . 5 per cent, sugar solution (dextrose, pentose, cane sugar, lactose) in a test tube add a small piece of yeast. Shake to make uniform mixtures, and with each fill a "fermentation tube. " Substitute water for sugar solution in a control test, and set the tubes aside in a warm place over night. Record the results. 10. Benedict's Method for the Determination of Sugar. Pre- pare 500 c. C. Of Benedict's solution as follows: Dissolve 9 g. Pure copper sulphate in a 500 c. C. Volumetric flask ...with about 100 c. C. Distilled water. Dissolve 50 g. Anhydrous sodic carbonate, 100 g. Sodium citrate, and 50 g. Sodium sulphocyanate in 250 c. C. Distilled water. The copper sulphate must be weighed ac- curately on the analytical balance. Pour the copper solution, 53 slowly, with stirring and without loss of a single drop, into the alkaline citrate solution. Then pour the mixed solution back into the measuring flask without loss, add 5 c. C. 5 per cent, potassium ferrocyanid solution, and with the rinsings make the total volume up to 500 c.
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