Laboratory Manual of Biological Chemistry With Supplement
Laboratory Manual of Biological Chemistry With Supplement
Otto Knute Folin
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5 and divided by the reading of the un- known (in m. M. ) gives the acetone content (in mg. ) of the vol- ume of urine taken for the analysis. Qualitative Test for Diacetic Acid (in traces). To 5 c. C. Of urine in a test tube add 1-2 c. C. Dilute acetic acid (10 per cent. ) and a small crystal of sodium nitroprussid. Shake a few times to dissolve the salt, then add an excess of concentrated ammonia (2-3 c. C. ), and mix. A violet color indicates diacetic acid. Gerhardt's Ferric Chlorid Test for... Diacetic Acid. This test is useful for showing the presence in urine of relatively large amounts of diacetic acid. It is made as follows : To 5 c - c - f urine in a test tube add ferric chlorid solution (10 per cent. ), drop by drop. At first a white precipitate of ferric phosphate is obtained, then, as the addition of the reagent is continued, a dark red color is produced if diacetic acid is present (in more than traces). A number of substances used as drugs, such as salicylic acid, phenacetin, etc.
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