Lipoprotein Structure : Apoprotein Interactions in Human Plasma High Density Lipoproteins

Cover Lipoprotein Structure : Apoprotein Interactions in Human Plasma High Density Lipoproteins
Lipoprotein Structure : Apoprotein Interactions in Human Plasma High Density Lipoproteins
Grow, Thomas Ellis
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^ t-H 1 •u 0) C/3 rO T1 (ti ^ c rH W CO •H -C S CO O (U •H C c; jn O •H > -H tH 0) ^J C MQ cn-H o i-C hJ ■w 0) 1 ;^ c tJ r-.
• ij JJ •rl ■H M e^n 14-J T3 T1 CflCN C2 fIJ CU rHr-i M CJ >.'— ' W H •H ^ 1 CJ (U H u CO . 6 1 C CU M O C/1 cfl V( O M Q U 0) P^ m CiO u IS 102 o ®; '"^l o w aons H3d Hda 103 removal of Sepharose. Again, all three types of labeled apoproteins are present. The only possible source of label is by exchange with labeled HDL bound to the Sepharose.
Incorporation of Labeled Li
...pid into HDL ^ and HDL - To study lipid exchange in lipoproteins it is first necessary to incorporate labeled lipid into one of the lipoprotein subclasses. As most lipids are quite insoluble in aqueous solutions and because one must be able to separate any free lipid from the labeled HDL, it is desirable to place the lipid on a solid support that can be easily separated from the solution. Because the physical state of the lipids appears to be important in determining a lipid binding protein's ability to incorporate them (94), the lipid was first adsorbed to celite parti- cles before mixing with the HDL to be labeled.

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