Louis Pasteur His Life And Labours

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Up to that point, the resemblance between the microbe of splenic fever and the microbe of cholera is complete. But this blood, or the cultures exposed to the free contact of the air, instead of continuing this first mode of generation, frequently exhibit, even in the course of twenty- four hours, spores distributed more or less regularly along the length of the fila- ments. All around these corpuscles the matter of the filaments is absorbed, in the manner formerly illustrated by Pasteur in the ...diagrams of his work on the diseases of silkworms, when treating of the bacilli of putrefac- tion. Little by little, all cohesion between the spores disappears, and the whole collection soon forms no- thing more than a dust of germs. But- and here lies the great difficulty which experimenters encountered in applying to splenic fever the method of gradual THE VACCINE OF SPLENIC FEVER. 235 attenuation which was practised with the microbe of cholera these germs of splenic fever may be exposed for years to the air without losing their virulence, always ready to reproduce themselves without any appreciable change, and to manifest their effects in the bodies of animals.

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