Manipulation of the Microscope

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If the greater expansion or coma is when the object is without the focus or farthest from the objective, the lenses must be placed farther assunder (or opened). If the greater coma is when the object is within the focus, or nearest the objec- tive, the lenses must be brought closer together ( or closed ). When the objective is in proper adjustment, the expansion of the outline is the- same both within and without the focus. " liiiniersioii-Adjustable Objectives. — As was stated before, immersio...n contact between the objective and cover glass is made by either water or homo- geneous fluid. The fluid should be kept in a small bottle or phial, the cork of . Which is pierced to receive a small pointed stick or match, and this should pro- ject sufficiently so that it will enter the fluid about J-inch. The fluid will then always be free from dust, and by withdrawing the cork the stick will always carry a drop of fluid with it.
In fixing an immersion objective to the stand, the latter should first be put in an upright position; the fluid should now be attached to the front lens, but care should be taken not to put on too much; it should be merely enough to cover the surface.


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