Methods And Formulae Used in the Preparation of Animal And Vegetable Tissues for
Methods And Formulae Used in the Preparation of Animal And Vegetable Tissues for
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Alcohol until they are a pale yellow, dehydrated, cleared, and mounted in Balsam.
The sections are frequently counterstained with Eosine or Vesuvine.
Weigert's Modification of Gram's Method.
As the prolonged washing in Alcohol may remove colour from the organisms, Weigert proposed the substitution of Aniline for Alcohol, and conducts the process on a slide. Place the section on a glass slide, and stain it with a few drops of Gentian- Violet Aniline-Water ; remove the excess of fluid, and apply ...for 2 minutes a few drops of Gram's solu- tion. Eemove this by gently blotting it off ; then wash the section by allowing Aniline to flow gently backwards and forwards over it'; when no more colour comes away, pour off MICRO-ORGANISMS. 65 the Aniline, and treat in a similar way with Xylol for about a minute, and mount in Balsam.
Previous to this process for bacteria, the nuclei in the sec- tions may be stained with Carmine (p. 28).
Kuline's Modification of Gram's Method.
Kiihne uses Fluorescine Alcohol after Gram, in the place of Alcohol for washing, and adopts Weigert's Aniline for clear- ing ; he also stains the nuclei with Carmine (nuclear staining, p.
What to read after Methods And Formulae Used in the Preparation of Animal And Vegetable Tissues for?
You can find similar books in the "Read Also" column, or choose other free books by Peter Wyatt Squire to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
Alcohol until they are a pale yellow, dehydrated, cleared, and mounted in Balsam.
The sections are frequently counterstained with Eosine or Vesuvine.
Weigert's Modification of Gram's Method.
As the prolonged washing in Alcohol may remove colour from the organisms, Weigert proposed the substitution of Aniline for Alcohol, and conducts the process on a slide. Place the section on a glass slide, and stain it with a few drops of Gentian- Violet Aniline-Water ; remove the excess of fluid, and apply ...for 2 minutes a few drops of Gram's solu- tion. Eemove this by gently blotting it off ; then wash the section by allowing Aniline to flow gently backwards and forwards over it'; when no more colour comes away, pour off MICRO-ORGANISMS. 65 the Aniline, and treat in a similar way with Xylol for about a minute, and mount in Balsam.
Previous to this process for bacteria, the nuclei in the sec- tions may be stained with Carmine (p. 28).
Kuline's Modification of Gram's Method.
Kiihne uses Fluorescine Alcohol after Gram, in the place of Alcohol for washing, and adopts Weigert's Aniline for clear- ing ; he also stains the nuclei with Carmine (nuclear staining, p.
What to read after Methods And Formulae Used in the Preparation of Animal And Vegetable Tissues for?
You can find similar books in the "Read Also" column, or choose other free books by Peter Wyatt Squire to read onlineMoreLess
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