The book Methods in Plant Histology was written by author Charles Joseph Chamberlain Here you can read free online of Methods in Plant Histology book, rate and share your impressions in comments. If you don't know what to write, just answer the question: Why is Methods in Plant Histology a good or bad book?
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In the cell of a root-tip a series of small canals, or vacuoles, appears, which is much more definite and extensive than the usual display of vacuoles and which appears before any vacuoles can be recognized in preparations made in the usual way (Fig. 24, B). Being a zoologist, he has called these vacuoles canaliculi. Bensley's Fixing Agent. 1. Formalin (neutral) 10. C. C. 2. Bichromate of potash 2. 5 g. 3. Corrosive sublimate 5. G. 4. Water 90. C. C. Dissolve the bichromate of potash in the wat...er, then add the corrosive sublimate and finally add the formalin. The solution 2, 3, 4 will keep, but the formalin soon becomes acid. Add the formalin to 2, 3, 4 only when needed for use. Obtain the neutral formalin by distilling the ordinary commercial formalin. Proceed as follows: 1. Fix 24 hours. 2. Wash in water, 24 hours. 3. Iodize on the slide. 4. Wash in water, 5 minutes. 5. Copper acetate (neutral) saturated solution in water, 5 to 10 minutes. 6. Wash in water, 1 minute. 7. ^ per cent haematoxylin, 5 to 10 minutes.
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