Micro-Organisms And Disease. An Introduction to the Study of Specific Micro-Organisms

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By careful washing the point may be so hit off that the spores appear deeply stained, whereas the bacillary substance is only faintly so.
The finest specimens are obtained by boiling the dried film specimen in carbol fuchsin ; then wash well in water ; then place the specimen in methyl-blue anilin water for half a minute to one minute ; wash again well ; dry and mount in xylol balsam : the bacillary substance appears blue, the -spores bright red.
It has been shown by Engelmann that the presence
... and renewal of oxygen as well as a certain concentration of the nutritive material are essential for the motihty of those bacteria that are possessed of cilia, i.e. that are possessed of locomo- tion and which normally grow aerobically. This, of course, does not apply to the motile anaerobic bacilli, e.g. bacillus of malignant oedema, tetanus, or butyricus.
As long as the bacteria are living, their protoplasm does not combine (stain) with nitrate of silver solution, only after death does this become possible.


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