Milk And the Public Health
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To prepare : Five grammes each of sodium taurocholate and lactose, 20 grammes of peptone, and 1 litre of water are heated together until the solids are dissolved. The mixture is filtered and sufficient strong neutral litmus solution is added to give a distinct colour. The medium is then distributed into fermentation tubes, and sterilised, by steaming for 20 minutes on three successive days.
Definite fractions of the milk are added by pipette to these tubes. The amount to add will depend upon th...e suspected degree of pollution of the milk. A usual procedure is to add I/O, O'l, O'Ol c. C. If the sample is byre milk, and if it is ordinary vended milk to add in addition O'OOl, O'OOOl, 0-00001, 0-000001, and occasionally even 0-0000001 c. C. The dilutions are obtained in the ordinary way, as described in Chapter IX.
These dilutions are widely spaced, and consequently the number of B. Coli present can only be enumerated between rather wide limits. The liability to accidental differences from faulty or unequal dilution is not inconsiderable.
What to read after Milk And the Public Health?
You can find similar books in the "Read Also" column, or choose other free books by William George Savage to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
To prepare : Five grammes each of sodium taurocholate and lactose, 20 grammes of peptone, and 1 litre of water are heated together until the solids are dissolved. The mixture is filtered and sufficient strong neutral litmus solution is added to give a distinct colour. The medium is then distributed into fermentation tubes, and sterilised, by steaming for 20 minutes on three successive days.
Definite fractions of the milk are added by pipette to these tubes. The amount to add will depend upon th...e suspected degree of pollution of the milk. A usual procedure is to add I/O, O'l, O'Ol c. C. If the sample is byre milk, and if it is ordinary vended milk to add in addition O'OOl, O'OOOl, 0-00001, 0-000001, and occasionally even 0-0000001 c. C. The dilutions are obtained in the ordinary way, as described in Chapter IX.
These dilutions are widely spaced, and consequently the number of B. Coli present can only be enumerated between rather wide limits. The liability to accidental differences from faulty or unequal dilution is not inconsiderable.
What to read after Milk And the Public Health?
You can find similar books in the "Read Also" column, or choose other free books by William George Savage to read onlineMoreLess
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