Neurological Technique; Some Special Histological Methods Employed for the Study of the Nervous System, Together With a Laboratory Outline for the Dissection of the Central Nervous System And the Neurological Nomenclature "[bna]" Arranged in a Classified

Cover Neurological Technique; Some Special Histological Methods Employed for the Study of the Nervous System, Together With a Laboratory Outline for the Dissection of the Central Nervous System And the Neurological Nomenclature "[bna]" Arranged in a Classified
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2. Cut out the region from which sections are desired and wash in several changes of distilled water, 4-8 hours, 3. Dehydrate with alcohol, imbed in celloidin, and section as directed in X, 3, 4, and 5, p. 64. Make sections about 15 /* thick. If serial sections are desired, proceed as in XI, p. 72.
4. To stain. — Withdraw 70 per cent, alcohol from the sec- tions and replace it with 50 per cent, for about 5 minutes. Then replace 50 per cent, alcohol with the aniline-blue solution (B) for 20-60 m
...inutes.
5. To differentiate. — {a) Rinse in 2 or 3 changes of distilled water for not more than 5 minutes. The sections are now a blue-black.
((5) Then transfer the sections, one at a time, to a shallow dish of 95 per cent, alcohol, to every 30 cc. of which there has been added 2 cc. of the alcoholic i per cent, caustic potash solu- tion (D). Agitate the fluid about the section till it acquires a light brownish-red color. This usually takes from 1-3 minutes.
If it takes more than 5 minutes, either the section is too thick or a few more drops of the alkaline alcohol are necessary.


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