Notes On the Methods Employed in Biological Studies

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For an interesting account of the reactions of living matter to very dilute solutions of certain substances whicli are poisonous when used in greater strength, see Leow and Bokomy. These investigators use a dilute alkaline solution of argentic nitrate in the discrimination between living and dead protoplasm; upon application of the reagent the former turns black, the latter remains uncolored. The solution is made by mixing 1 cc. of a 1 per cent, solution of the nitrate in distilled water A\ith
...an equal amount of a solution containing 13 parts of potassic hydrate so- lution, 10 parts of ammonia, and 77 parts of distilled water.
Chloride of Mercury (Corrosive Sublimate).
Mercuric chloride, or Corrosive sublimate, dissolved in fifty parts of absolute alcohol, renders protein grains insoluble in waterj Pfeffer recommeniis that the specimen should remain in this reagent at least twelve hours. Dippel uses a dilute aque- ous solution (1 in 500) to render visible the currents in the most delicate threads of protoplasm (and for the demonstration of the nucleus without affecting the other contents of the cell).


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