Notes On Two "atoxic" Strains of Diptheria Bacilli

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Result.
1656 1703 ....
■929 2S0 257 2S7 S s s J4 1/20 No lesion.
Large sloug-h, ulcer, paralysis.
This table shows that one-twentieth antitoxic unit com- pletely neutralizes the pathological action of five cubic centi- meters of the culture filtrate.
Culture No. 50. — The unfiltered fluid from cultures even in doses of five cubic centimeters produced only a moderate to heavy edema, frequently leading to the formation of small subcutaneous nodules but not to necrosis. The injection of the filtra
...te in doses of five cubic centimeters produced edema and induration which persisted in the form of small nodules for a time. More rarely, superficial necrosis or scab-forma- tion followed the edema. The loss in weight was nominal as Table V. indicates. The more severe effect of the filtrate may be referred to the longer incubation of the culture.
The few offspring that were available for subsequent immunity tests possessed no greater than normal resistance to the L + dose of the toxin-antitoxin mixtures as shown in the following table : Digitized by Microsoft® TWO " ATOXIC " STRAINS OF DIPHTHERIA BACILLI.


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