On the Photochemistry of the Retina And On Visual Purple

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As far as I can see, there is still another but less trustworthy method, namely, to carry out the experiment on several frogs, and to examine the one set immediately, the other not until they have been kept some time in the dark. If these are the experiments on which Boll's statements rest, they may be confirmed by slxxj one who 1 Bericht. d. Berl. Acad. , 26 Nov. 1877.
II.] VISUAL PURPLE. 63 cares to repeat them, paying attention meanwhile to the follow- ing facts.
Even the extirpated uninjure
...d frog's eye needs a large amount of light in order to manifest even traces of change in its visual purple, much more so is the eye still in connection with its owner, that is, intra vitam. Without direct sunlight, I have never succeeded in a room in obtaining frogs' retinas free from visual purple, not even when I allowed the animals to leap about before the window the whole day long, whether under a glass or uncovered and only secured by a long thread. Only when I treated the animal in the same way in the open air (in January and February) was I successful ; and even then not always, as is intelligible when it is remembered that many frogs know how to protect themselves against an injurious light by drawing in their eyes and closing their nictitating membrane.

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