On the Question of the Occurrence of Creatinine And Creatine in Blood

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On the Question of the Occurrence of Creatinine And Creatine in Blood
Helen Jeanette Allen Behre
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Yet after treatment to convert creatine into creatinine, it is possible by means of the action of alkali or of kaolin, to separate practically quantitatively the creat- inine derived from creatine from the chromogenic substance originally present in the blood. Typical results in this connection are presented in Tables IV and V.
The results recorded in Tables IV and V serve to show how dif- ferently the creatine of normal blood behaves after conversion to creatinine as compared with the chromoge
...nic substance originally present in the blood. The remarkably sharp separations effected by means of alkali or of kaolin are very striking, and serve to further substantiate our earlier conclusion that preformed crea- tinine does not exist in blood.
The view that creatine occurs in blood in very appreciable amounts under certain conditions finds confirmation in an isola- tion experiment, in which creatine was isolated as creatinine-zinc chloride. In this experiment the blood obtained from a dog 46 hours after ligation of both ureters was employed.


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