Papers And Addresses

Cover Papers And Addresses
Papers And Addresses
Welch, William Henry, 1850-1934
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The silk sutures when removed were proven both by microscopical examination and by roll or plate cultures to contain with great regularity the white staphylococcus, often in considerable number, often enclosed within leucocytes, and this not only where a stitch abscess had formed but also where there was not a trace of suppuration or visible reaction around the stitch.
A crucial experiment is the following: The skin is thoroughly disin- fected, in the manner to be presently described, so that c
...ulture tubes of nutrient agar or gelatin inoculated by scrapings from its surface remain sterile. A silk thread, sterilized by steam and proven by culture methods Digitized by Google THE INFECTION OP WOUNDS 411 to be sterile, is passed one or more times by means of a sterilized instrument through the skin, is withdrawn, and at once a tube of melted nutrient agar is inoculated with the thread and rolled. This amounts to a ready means of making cultures directly from the deeper layers of the epidermis and the skin, and is a method applicable for many purposes to the bacteriological examination of the skin.

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