Pathogenic Micro-Organisms; a Text-Book of Microbiology for Physicians And Students of Medicine
Pathogenic Micro-Organisms; a Text-Book of Microbiology for Physicians And Students of Medicine
Ward J Macneal
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Dilution-cultures prepared as described in the next paragraph, where the principle is the same, are shown in Fig. 43. In tube No. i the colonies are so numerous as to look like fine white dust. In tubes 2 and 3 they become less numerous and larger. Esmarch's Roll-tubes.— Use liquefied gelatin or agar. The dilutions in tubes i, 2 and 3 are made as above."^ Tubes contain- FiG. 42. — Manner of making Esmarch roll- tube. ing a rather small amount of the culture-medium are more con- venient. A block... of ice should be at hand, and, with a tube filled with hot water and lying horizontally, a hollow of the size of the test-tube should be melted on the upper surface of the ice. In this hollow, place the tube of liquefied gelatin or agar; roll it rapid- ly with the hand, taking care that the culture-medium does not run toward the neck as far as the cotton plug. The medium is spread in a uniform manner around the inside of the tube, where THE CULTIVATION OF MICRO-ORGANISMS III it becomes solidified.
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