Pathogenic Micro Organisms Including Bacteria And Protozoa a Practical Manual

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Examine hanging drop for form, size, and motility (and arrange- ment).
9. Make stained cover-glass preparations and examine.
10. Then try indol reaction with the same tubes.
11. While these tubes are incubating use material from the suspected colonies on the plates (1 and 5) for hanging-drop cultures.
12. Meanwhile make stained cover-glass preparations from other colonies of suspected cholera on the plates (1 and 5).
13. Make gelatin-tube cultures from colonies on plates (1 and 5).
14. Make gel
...atin-tube cultures daily for five or six days, to study shape of growth along the line of puncture to preserve the culture.
1 These direct microscopic examinations of the intestinal contents are, as a rule, very unsatis- factory, at least in those in which the symptoms are not marked. In a few the spirals will make up from . 50 to 100 per cent, of the bacteria present. In most of the cases during the last epidemic in New York Dunham found abundance of columnar epithelium from the intestinal mucous mem- brane, numerous straight, thick bacilli, and only a few curved bacilli or segments of spirals too few to identify.


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