Physical Chemistry for Physicians And Biologists

Cover Physical Chemistry for Physicians And Biologists
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The latter were con- tained in glass dishes (with ground covers) kept in a thermostat at 18°.
After the solution had acted for a certain time, the action of the germicidal agent was stopped (according to Gep- pert's method), by rinsing the garnets in water and treat- ing them with such reagents as converted the germicidal substances into neutral, non-germicidal substances. So, for example, the salts of the heavy metals were precipi- tated by a four per cent ammonium sulphide solution; acids and
... bases were neutraUsed, etc.
After again washing in water, five garnets each were * Those who are acquainted with the method of bacteriolop}' will understand how the phrase "equal number" is to be inter* preted.
Digitized by Google 238 PHYSICAL CHEMISTRY, introduced into test-tubes containing 3 c.c. of water.
The tubes were shaken for three minutes, whereby the spores were detached from the garnets and distributed through the water.
The emulsion of spores prepared in this way was heated to 37.5° and added, with stirring, to 10 c.c.


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