Physiological Chemistry volume 2

Cover Physiological Chemistry volume 2
Physiological Chemistry volume 2
Karl Gotthelf Lehmann
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As a matter of course, we only employ for this purpose the solid residue, after thorough drying at J20. The best method of procedure is to introduce into a small digesting flask the dry substance which is to be employed for the determination of the fat, while we determine its weight, as in elementary analyses, by re-weighing. A small digesting flask is necessary for this purpose, since it is only thus that we can boil the substance with ether, and pour off the ethereal fatty solution without lo...ss of fat. The ethereal solution is then to be evaporated from a small glass cup or basin with a very high border, because the fat very readily creeps up to the edges, and thus necessarily occasions loss. Moreover, the ether must be perfectly pure, as free as possible from water, alcohol, and free acid. The evapo- ration of the ether must be accomplished without boiling; and the fatty residue, like all other residues, be dried at 120.
Pure ether extracts only the neutral fats and the free fatty acids, and riot the alkaline sails of the fatty acids; the latter must be extracted with absolute alcohol, to which about one-tenth of its volume of ether has been added.


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