Practical Bacteriology Blood Work And Animal Parasitology Including Bacteriolo
Practical Bacteriology Blood Work And Animal Parasitology Including Bacteriolo
E R Edward Rhodes Stitt
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(There being 5, 000, 000 red cells to the cubic millimeter. ) As one cubic centimeter is 1, 000 times greater than i cubic millimeter, there would be 15, 000, 000, 000 bacteria in each cubic centimeter of such an emulsion, or vaccine, as it is termed. The standardization may be made with a haemacytometer. * Having determined the strength of the stock vaccine, we should pre- pare a dilute vaccine for injection. This is most conveniently carried out by filling vials with 50 c. C. Of salt solution..., plugging with cotton, then sterilizing in the autoclave. A sterile rubber cap is now drawn over the mouth of the vial. Sterility is insured by plunging the rubber cap and neck in boiling water. If the stock vaccine showed 5, 000, 000, - ooo bacteria per c. C. And we desired to have a vaccine containing 200, - 000, 000 bacteria per c. C. , it would only be necessary to draw out 2 c. C. Of the salt solution by means of a sterile syringe needle inserted through the rubber cap and replace it with 2 c.
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