Practical Bacteriology Microbiology And Serum Therapy Medical And Veterinary
Practical Bacteriology Microbiology And Serum Therapy Medical And Veterinary
Albert Besson
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When the animal is dead aspirate a little of the heart blood, or take a little of the medulla from one of the long bones being careful to avoid contaminating it and sow tubes of agar and broth. Incubate at 37 C. Inoculation for the purpose of identifying a suspected pneumococcus ought always to be made into a mouse and not into a rabbit. Rabbits are not so susceptible to the disease as mice, and indeed in some cases of pneumococcal pneumonia the sputum contains pneumococci almost avirulent for ...rabbits (Gamaleia). (b) The inflammatory exudate. Collect the exudate by putting a syringe needle into an hepatized area (p. 198). The material must be examined by microscopical examination and by inoculation. Bezan9on and Griffon prefer to sow the exudate in a young rabbit's serum which has not been coagulated and to inoculate mice with the culture obtained after incubating for 21 hours. (c) Pus and inflammatory exudates in other situations. The technique is the same as for the material from the lung.
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