Practical Biological Chemistry Guide Pour Les Manipulations De Chimie Biologiq

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Practical Biological Chemistry Guide Pour Les Manipulations De Chimie Biologiq
Gabriel Mile Bertrand
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Heat to boiling in a beaker 125-130 cc. Of water and pour the suspension of starch into it, stirring thoroughly meanwhile. The total volume should be made up to 150 cc.
132. Conversion of Starch to Sugar. Three cubic centi- metres of hydrochloric acid (at 22 B. ) are added to the above paste, which is thoroughly stirred and brought to boiling. Previous to performing this hydrolysis, pipette off about i cc. Of the liquid, put it in a test-tube with 10 cc. Of water, and add 2 or 3 drops of iodine
... solution, 1 when the fine blue colour of iodide of starch will appear. While the boiling is in progress remove successive small quantities every three or four minutes and test it in the same manner. A series of tints will thus be obtained : blue, violet, reddish violet, red, mahogany, and chestnut. When the liquid gives no colour with iodine (after about 25 to 30 minutes), the boiling is stopped, and a further quantity of the solution tested with alkali-copper solution, which will be strongly reduced.

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