Practical Methods in Microscopy

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Practical Methods in Microscopy
Charles H Charles Herbert Clark
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Then pour in the picric acid solution slowly. This will pro- duce a precipitate which will in time nearly but not quite all dissolve. If a slight precipitate does not persist, add a little more of the picric acid solution, and filter several times. Object should be soaked in water before staining and washed out in water after staining.
8. Orth's Lithium Carmine.
Saturated aqueous solution of lithium carbonate . 100 c. C. Carmine 2 grammes Sections may be passed from water into this stain. Exces
...s of stain may be removed by washing in acid alcohol (70 per cent alcohol, 100 c. C. ; hydrochloric acid, 1 c. C. ). Thus used, this is a good nuclear stain. Without the washing in acid alcohol, the stain is quite diffuse. This stain is highly recommended. Good double staining results are obtained by following this stain with an aqueous solution of picric acid. Sections stained in lithium carmine cannot be fixed to the slide with albumen fixative.
232 PRACTICAL METHODS IN MICROSCOPY.
9. Mayer's Cochineal Tincture.


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