Practical Physiological Chemistry a book Designed for Use in Courses in Practi
Practical Physiological Chemistry a book Designed for Use in Courses in Practi
Philip Bovier Hawk
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3 McLean and Van Slyke: Jour. Biol. Chem. , 21, 361, 1915. 3 Because of the difficulty or impossibility of obtaining Merck's blood charcoal, two modifications of this method have been suggested for the removal of protein. Harding and Mason (Jour. Biol. Chem. , 31, 55, 1917) precipitate the protein by adding copper sulphate and alkali, while Foster (Jour. Biol. Chem. , 31, 483, 1917) proposes the use of metaphosphoric acid. The McLean and Van Slyke procedure is followed after the protein removal... in each case. 4 Merck's " Blood Charcoal Reagent, " purified by acid and free from chloride, is used. PHYSIOLOGICAL CHEMISTRY iodide. 1 The titration is performed in the presence of nitrous acid and starch, so that the first drop of iodide in excess of the silver present is changed to free iodine and gives the blue starch-iodine color. The optimum acidity for the end point is fixed by the addition of trisodium citrate in amount equivalent (M mol. ) to the free nitric acid present. Under these conditions i drop of excess N/SO iodide gives a color perceptible in 150 c.
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