Practical Physiological Chemistry : a book Designed for Use in Courses in Practical Physiological Chemistry in Schools of Medicine And of Science
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Allow to stand over night. A heavy precipitate of magnesium ammonium phosphate should be found in the test experiment indicating that the phosphoric acid of the nucleic acid had been Uberated by the nucleotidase of the intestinal extract. The control should show only a sUght precipitate.
If desired the phosphorus of the precipitates may be determined quantitatively by dissolving in 2 per cent HNO3, precipitating as the phosphomolybdate and determining volumetrically according to the Neumann pro...cedure (see 554).
Experiments on Erepsin 1. Preparation of Erepsin. — Grind the mucous membrane of the small intes- tine of a cat, dog, or pig with sand in a mortar. Treat the finely divided membrane with toluol- or chloroform-water and permit the mixture to stand, with occasional shaking, for 24-72 hours. ^ Filter the extract thus prepared through cotton and use the filtrate in the following experiment.
2. Demonstration of Erepsin. — To about 5 c.c. of a i per cent solution of Witte's peptone in a test-tube add about i c.c.
What to read after Practical Physiological Chemistry : a book Designed for Use in Courses in Practical Physiological Chemistry in Schools of Medicine And of Science?
You can find similar books in the "Read Also" column, or choose other free books by Hawk, Philip B. (Philip Bovier), B. 1874 to read onlineMoreLess
To quickly assess the difficulty of the text, read a short excerpt:
Allow to stand over night. A heavy precipitate of magnesium ammonium phosphate should be found in the test experiment indicating that the phosphoric acid of the nucleic acid had been Uberated by the nucleotidase of the intestinal extract. The control should show only a sUght precipitate.
If desired the phosphorus of the precipitates may be determined quantitatively by dissolving in 2 per cent HNO3, precipitating as the phosphomolybdate and determining volumetrically according to the Neumann pro...cedure (see 554).
Experiments on Erepsin 1. Preparation of Erepsin. — Grind the mucous membrane of the small intes- tine of a cat, dog, or pig with sand in a mortar. Treat the finely divided membrane with toluol- or chloroform-water and permit the mixture to stand, with occasional shaking, for 24-72 hours. ^ Filter the extract thus prepared through cotton and use the filtrate in the following experiment.
2. Demonstration of Erepsin. — To about 5 c.c. of a i per cent solution of Witte's peptone in a test-tube add about i c.c.
What to read after Practical Physiological Chemistry : a book Designed for Use in Courses in Practical Physiological Chemistry in Schools of Medicine And of Science?
You can find similar books in the "Read Also" column, or choose other free books by Hawk, Philip B. (Philip Bovier), B. 1874 to read onlineMoreLess
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