Preservation of Bull Semen At Sub Zero Temperatures
Preservation of Bull Semen At Sub Zero Temperatures
N L Vandemark
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0 46. 5 44. 3 45. 3 15. 5 2 41 38 38 39. 1 6 42 45 43 43. 6 18 42 43 42 42. 5 Average 42. 0 41. 8 41. 4 41. 7 20 BULLETIN No. 621 [October, reduced with each rise in temperature. Thus, it appears that cooling to refrigerator temperature (4-5 C. ) before adding the glycerol should be a part of the routine procedure. A comparison of the results from adding the glycerol in 5, 3, and 1 equal portions is given also in Table 11. Little difference in survival during freezing was noted between the thre...e rates of addition. Using 3 equal additions resulted in slightly better results, but the advantage was not statistically significant. While little difference was evident from adding the glycerol in 3 portions as compared to 1, many still use 3 additions in the hope of obtaining a slightly better sperm sur- vival. In fact, some have gone to a procedure of adding the glycerol dropwise with constant gentle agitation. This method has not been tested in this laboratory. Allowing sperm to equilibrate with the glycerol.
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