Preventive Medicine And Hygiene

Cover Preventive Medicine And Hygiene
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This is cen- trifuged for 10 minutes at a speed of from 2,000 to 3,000 revolutions per minute. The sediment upon the rubber stopper of the distal end 526 ANIMAL FOODS of the tube is mixed with a drop or two of water and spread upon a slide in a thin even layer, covering a space of about four square centi- meters. This is dried and stained with methylene blue. The micro- scopic examination reveals the character of the milk as judged from the approximate number of pus cells and presence of strept...ococci in long chains. It has been found that the number of cocci, bacilli, or chains in the 1/12 oil immersion field, multiplied by 10,000, gives a rough approximation of the number of bacteria in a cubic centimeter of the whole milk.
The results of this method vary considerably with details of in- dividual manipulation, with the speed of the centrifugal machine, with the time allowed for centrifugation, and other factors.
(2) The Doane-Buckley Method. — In this method the number of leukocytes are counted in the chamber of the Zeiss blood counter, which contains just 0.0001 c.


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