Proceedings of the New York Pathological Society, volume 2
Proceedings of the New York Pathological Society, volume 2
New York Pathological Society
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Libman, serum agar plus glucose (^^^ to 2%). Maximum growth was obtained with the higher per- centage of glucose. With the addition of still higher Digitized by VjOOQ IC 130 n. 1,. CKLLER, M.D. percentages of glucose (3, 4, 5%) as well as with the use of lactose of sacharose (2%) the development of the organism was neither furthered nor hindered. On Loeff- ler s serum there is an abundant growth, but there is no advantage over the serum glucose media just described. On glycerine agar the growth... varies, usually only a few fine colonies being obtained; but on the addition of serum the growth is profuse. In fluid media the organism grows characteristically. The growth begins as a diffuse cloudiness near the sur- face that after two or three days forms a white scum on the surface. The remainder of the medium is at first clear, later slightly clouded, while at the bottom of the tube there is a floccular sediment. There is considerable difficulty in obtaining growth in this form as the shaking of the tube will prevent the formation of the scum or cause it to break up and fall to the bottom after it has formed The majority of our cultures have grown slightly in ordinary bouillon with and without the addition of sugar in the form of glucose, lactose, or saccharose.
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