Proceedings of the Section of Sciences, volume 10

Cover Proceedings of the Section of Sciences, volume 10
Proceedings of the Section of Sciences, volume 10
Koninklijke Akademie Van Wetenschappen (Netherlands). Afdeeling Natuurkunde
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By means of a pipette 3 cc. of liquid agar-agar were put into each little cylinder. I shall not discuss the way to prepare this liquid : it is to be found in ail handbooks on the technics of bacteriology. It must be observed, however, that it is advisable to let the agar-agar solution cool down to ± 45® before measuring it in the pipette; otherwise there is a danger of its flowing partly away from under- neath the glass cylinder.
After some time the agar colums have become solid and are placed,
... still surrounded by the glass tube, on the spread out parts of the membrane which, if necessary, has been previously cleaned. For this cleaning which also may serve to remove the mucus, we take NaCl 0,97». Investigations especially made for this purpose on the gastric mucous membrane have shown, however, that for this organ at least washing with water gives satisfactory results.
On the mucous membrane, which if necessary has been cleaned, the agar-agar colums remain for 8 hours or more, in order to enable enzymes and pro-enzymes to diffuse into the agar-agar.


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