Proceedings of the Society for Experimental Biology And Medicine
Proceedings of the Society for Experimental Biology And Medicine
Society for Experimental Biology And Medicine (New York, N.Y.)
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Over fifty comparisons were made. In Oder to ascertain positively whether the modified method would produce ammonia from non-ammoniacal radicals, weighed samples of urea, uric acid, glycocoU, taurin, leucin, tyrosin and hippuric acid were added separately and also collectively to 25 c.c. portions of urine and the results compared with the figures for the ammonia obtained from equal volumes of the original urine. In no case was any increase obtained. The tests were repeated, but 20 c.c. of a sta...ndard solution of ammonium chloride were substi- tuted for the 25 c.c. of the urine. In these cases, also, no increase of ammonia output was obtained. As a final test of the eflSciency of the modified method the ammonia content was determined in a normal urine by both the Folin method and the modified method. Then to equal volumes of the original urine 0.5 gram samples of triple phosphate were added, and the ammonia contents again determined by both methods. The ammonia was also determined in separate portions of the triple phosphate.
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