Purification, Partial Characterization, And Serology of the Capsid And Cylindrical Inclusion Proteins of Four Isolates of Watermelon Mosaic Virus
Purification, Partial Characterization, And Serology of the Capsid And Cylindrical Inclusion Proteins of Four Isolates of Watermelon Mosaic Virus
Baum, Robert H.
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Reciprocal SDS-double immunodiffusion tests between WMV-1, WMV-2, and WMV-M, with antisera obtained during the first four months after initial injection of immunogen. Media contains 0.8% Noble agar, 0.5% SDS and 1% sodium azide. Center wells were charged with: lvs = WMV-1 Florida antiserum 2vs = WMV-2 Florida antiserum Mvs = WMV-M antiserum. Antigens in peripheral wells were as follows: WMV-1 Florida in sap WMV-1 Jordan in sap WMV-2 in sap WMV-M in sap corresponding purified virus preparation, ...50 yg/ml H = healthy pumpkin sap. e 05 tO rH 3 3 C 3 U O -H -H 01 to 3 u en O O "H -H 01 0) X! £ 4J to 3 JS •H AJ 3 -h 3 O T3 w pq C U 6 & 3 a 0) > - 3 s 3 4J IH S-i I 1 M 1 ■n eg _3 > 05 E 1) •u -J 2 H ■H ■3 I+-I 3 •H 3 i — i X >, 3 3 01 C 3 3 U 01 05 u jj 3 a T" c — ' •r-i M '~ 3. r-i -3 g 1) CO cd c . « x> u 4J £ ~ S 3 g ~ *- £ i M 14-1 3 V c > a u 4-1 X s : -i-i -J ■H 3 C >-. 3 rC a to H (J •3 3 to H — 1 >, 3 — tu 3 i — i X —i 4J u 0) •H i-i n cd -j 4J 4-1 3 1/ •H 3 1 N X 05 X2 £ 3 ■H 05 > -2 •r-t — g •H ^ cd X ^ 3 *— ' ij c « ;~ H M ■I) X 1 s u 0- r TZ • r- 3 > 3 -d O, t-H m >-i 3 3 H y- § u E 3 •- 1 a i+j TJ M •- i ■a g ■H c 11 C i_l U u U-, •H a •r-i C 3 U 3 3 Cd 3 ■H i •r-1 r-i •ui £ en y| J-J r-l •H U-l •-H 3 c 1 3 3 C -a 3 3 -H P.
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