Scientist And Patent Agent At Genentech Oral History Transcript 2002
Scientist And Patent Agent At Genentech Oral History Transcript 2002
Dennis G Kleid
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S. Patent 4, 777, 043, and VR Anicetti, SE Builder, BJ Marks, JR 152 keys was growing the cells in serum-free media. The major protein in the media was tPA. The next important step in the process used lysine-Sepharose. 1 tPA likes to bind to the lysine. So they developed a process using lysine-Sepharose columns. The media was poured through there and then eluted. That s called affinity chromatography. Wellcome used antibodies to do that step. They started with the serum-containing media . So th...ey had a lot more downstream steps than we did. Clinical Trials and Criticism Hughes: Talk about the relationship between Genentech, Elliot Grossbard, and the cardiologists who were testing tPA in the clinic. Kleid: Genentech and Elliot, with the help of several major cardiologists in the United States, developed the original tPA clinical trial protocol that we already talked about, where they tested tPA in people and designed the protocol that incorporated the urokinase treatment if the heart attack progressed.
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