Some Factors Influencing the Quantitative Determination of Gliadin

Cover Some Factors Influencing the Quantitative Determination of Gliadin
Some Factors Influencing the Quantitative Determination of Gliadin
J E Joseph Eames Greaves
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In the above results, however, the hard wheats show a slightly higher average ratio than do the soft wheats. This may be due to a higher per cent of optically active sugars in the hard wheats.
Inasmuch as the ratio of per cent nitrogen to polariscope reading is a constant* with varying ratios of alcohol to flour, and a greater amount of nitrogen is extracted when the ratio is 7. 985 grams of flour to 100 c. C. Of alcohol than when it is 15. 97 grams of flour to 100 c. C. Of alcohol, the former
...ratio has been used in the following work. That this does not decrease the accuracy of the polariscope method is shown by the following consideration. The dilute solutions are less colored than the more concentrated, and for this reason the error in the polari- scope reading is less in the dilute solution than the more concen- trated and darker one. An error of . 1 on the sugar scale of a * I. E. , in this work within experimental error. That the specific rota- tion of a great number of organic compounds does vary with the concen- tration of the solution is a well established fact.

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