Studies in the Nitrogen Metabolism of Bacteria

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Studies in the Nitrogen Metabolism of Bacteria
Harry Johnson Sears
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1 2. 0 71. 0 20. 1 3. 5 7 122. 0 58. 1 2. 3 92. 3 22. 7 4. 1 117. 5 55. 5 2. 1 97. 8 31. 1 3. 1 11 114. 0 62. 5 1. 8 129. 3 35. 0 3. 7 14 101. 2 68. 7 1. 5 112. 0 37. 8 3. 0 18 109. 0 65. 9 1. 7 135. 8 51. 2 2. 6 Chart 5. The formation by hacteri. N of amino-acid and ammonia from peptone.
Nitrogen Metabolism of Bacteria 23 That the presence of glucose might decrease the peptone-splitting power of the cultures by preventing, to some extent, the reproduction of the organisms is contrary to experi
...ence, and would furthermore make the identical ammonia curves difficult to explain.
The decrease during the first 24-48 hours in the amount of free ^ ammonia which was originally present in the culture medium is noticed in the case of all the organisms studied. This decrease seems to take place to practically the same extent in the cultures containing glucose. It is undoubtedly true that in such cultures the most rapid multiplica- tion of the organisms takes place during the first 24-48 hours. It is therefore probable that this ammonia is utilized for the synthesis of bacterial protein.


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