Text book of Physiological Chemistry in Thirty Lectures

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Almost all of the investigations in this direction have been made with defibrinated blood. In the first place, we are interested to know the relative amounts of blood-corpuscles and serum.
This varies with different kinds of animals, and even in different animals of one and the same species. Moreover, the estimation of the number of blood-corpuscles, and the amount of the serum, cannot be made very exactly. It is an indirect determination. We will briefly mention here that method upon which the
... figures that we shall give below have been based. It is that of Hoppe-Seyler.' The blood-corpuscles may be separated from the serum by means of the centrifuge. By repeatedly stirring the blood with an isotonic salt solution, and renewed centri- ' Handbuch der physiol. und pathol. chem. Analyse, p. 272 (1883).
THE BLOOD. 553 fugalizing, the serum lying between the separate blood-corpuscles is eventually removed. In these blood-corpuscles we can determine the sum of the hemoglobin and protein. If in addition the hemoglobin and albumin content of the total blood and the albumin content of the serum are determined, then from these values the relative amounts of serum and blood-corpuscles in the blood as a whole can be estimated.


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