The Methods of Bacteriological Investigation

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F., Bd. XX, 1879, S. 454.
t " Die Anagrobiosifrage." " Archiv f. d. ges. Physiologie," Bd.
XXSIII, 1883.
ITO BACTERIOLOOICAL mVESTIGATIOK gen from iron and sulphnric acid, and for the forma- tion of sulphate of iron free from the oxide. On this account, before the sealing in of the funnel-tube (a), Fio. 27.
A= a number of rolls of piano-wire are placed in A. B is used for the reception of about 10 c. cm. of a solution of prussiate of potash, and for the development of the double ferro-cyanide o
...f iron. O contains the nu- trient solution. The flasks are carefully cleaned, or, better, sterilized and dried by heat, and then at d joined together. After this, A is fiUed two thirds full with distilled water, rendered acid by a few drops of concentrated sulphuric acid, and heated to boiling. During the boiling care should be taken that the steam condensed in the tube (c?) between A and 5 flows back into A, by placing the apparatus in an in- clined position, because particles of the oxide of iron may be drawn into the flask B with the steam.

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