The Microtomists Vade Mecum a Handbook of the Methods of Microscopic Anatomy

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518 ; SQUIEE'S Methods and Formulse, &c, 1892, p. 35.
WEIGEET corrects unsatisfactory samples of picro-carmine in the following way (Virchow's Archiv, Bd. Ixxxiv, pp. 275, 315 ; Zool. Jahrb. , 1881, p. 40) : Small quantities of acetic acid are added " until the first slight precipitate appears even after stirring. " The whole is again put away for twenty -four hours more, when it will be found that there has formed a pre- cipitate that can only partially be removed by filtration ; ammonia is th
...en added drop by drop at intervals of twenty-four hours, until the solution becomes clear. If the solution stains too yellow, acetic acid is added ; if it overstains red, a little ammonia is again added. All badly staining samples of picro-carmine may, according to Weigert, be improved in the same way by addition of acetic acid or ammonia. I consider it to be a very happy-go- lucky process.
CARMINE AND COCHINEAL STAINS. 155 235. Lithium-Carmine (0TH, Berlin, klin. Wochenschr. , xxviii, 1883, p. 421).


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