The Mosaic Disease of Cucurbits

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The two methods of inoculation used differ cliiefly in the form of the inocu- lum, the expressed juice of mosaic plants or their crushed tissues being used. Results by either method have been successful, as ie shown below. Control inocuhitions were made in all cases, using caged plants and inoculating them with the expressed juices or crushed tissues of healthy plants (PI. IX, A).
TESTS WITH EXrnKSSED JUICES OF MOSAIC PLANTS.
In the earlier work the expressed juices of mosaic plants were used f
...or inoculation. Portions of a mosaic plant, cither leaves, stems, or fruits, were cut finely by means of a sterilized meat chopper, the juice pressed throuj^h clieesecloth, and in some cases filtered through liltcr ])aper, and the expressed juice used as the inoculum. The THE MOSAIC DISEASE OF CUCURBITS.
31 inoculations were made in the stems, leaves, and in some cases in the fruits. In the earlier experiments a sterile hypodermic needle was used for inoculation and the inoculum injected rather deeply into the stem.


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