The Nature of Enzyme Action

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If we make the reasonable assumption that the rate of change is in proportion to the amount of " compound " of enzyme and substrate in existence at the time, it will be seen that the result is what would be expected if this combination were of the 1 See also the criticism of Bratlsford Robertson's view by van Slyke (182).
59 6o THE NATURE OF ENZYME-ACTION nature of an adsorption-compound, since more trypsin will be in associa- tion with the substrate in proportion to the concentration of the en
...zyme when the latter is lower. The conditions in the first, linear, stage are not inconsistent with this interpretation ; in the case of congo-red and paper, when the amount of the dye is very small compared with that of the paper, practically all of the dye is taken up by the paper, since it is necessary to examine the solution in a deep layer in order to detect any colour in it ; such an amount would be within the errors of experiment in enzyme work.
The exponential law of activity of enzymes in relation to concentra- tion, of which the square-root law is a particular case, is the mathe- matical expression of the above facts.


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