The Ninth Component of Human Complement : a Study of Its Self-Association And Interaction With Lipid Bilayers

Cover The Ninth Component of Human Complement : a Study of Its Self-Association And Interaction With Lipid Bilayers
The Ninth Component of Human Complement : a Study of Its Self-Association And Interaction With Lipid Bilayers
Shiver, John W., 1957-
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In general, larger net currents resulted when larger voltages were applied, and the current continued to increase at all potentials throughout the course of the experiment.
C5b-9-mediated conductances . For the experiments described in this ection C5b-8 was added in quantities (1-3 ug of each protein in a 4.2 ml chamber) which did not produce significant conductances at a constant voltage of 40 mV. This was done in order to help prevent the bilayer from breaking due to excessive C5b-9 conductan
...ces. Initially, no changes occurred when C9 was introduced to a bilayer containing C5b-8 56 100 mV T 10 pA L 20 mV 100 mV F ig, lb . Voltage-dependent conductance changes of an asolectin planar lipid bilayer mediated by C5b-8. Both the cis and trans compartments contained 100 mM NaCl , 10 mM Tris, pH 1.1 solutions.
57 under these conditions. But within a few minutes conductances began which had different conductance characteristics from those observed with C5b-8 (see Figs. 16 and 17). Figure 16a shows discrete channels of widely different single conductances.


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