The Parasitic Amoebae of Man

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177 the contents of the abscesses containing amoebae. In order to control his experiments he believed it was necessary to cultivate the bacteria f ound in the feces of dysenteric patients and to inject these separately and together. Regarding these experiments he says: " Cultures were made from the feces of the same individuals whose discharges had been used to suc- cessively produce dysentery, and these were then in- jected into the intestines of four puppies. There was absolutely no effect pr...oduced. It, therefore, seems unreasonable to conclude that the germ that produces the disease is a bacterium; or, at any rate, it seems fairly certain that it cannot be an organism that develops, or even lives, in the culture media ordinarily employed. As neither of these suppositions appears at all probable, and as the amoeba was the only other living organism found in the feces, that was probably absent from the cultures, it seems logical to suppose that this parasite is the cause of any morbid state that the injection of these discharges may give rise to.

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