The Problems of Physiological And Pathological Chemistry of Metabolism, for Students, Physicians, Biologists And Chemists

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However, it has been learned that if the urine is treated with concentrated sulphuric acid as if for Kjeldahl estimation, and the fluid alkalinized after decolori- zatioh and distilled, alkylamine, including trimethylamine, passes over with the ammonia.*^ The author's student, Kinoshita,** proceeded as follows: He distilled off under lowered pressure the volatile amines from the urine in presence of magnesia, taking them up in a receiver containing dilute hydrochloric acid. In the chloride resi...due which remained after evaporation of the distillate, consisting mainly of ammonium chloride and a small admixture of the chlorides of other volatile bases, the amount of alkyl in nitrogen combination was determined by the method of J. Herzig and H. Meyer, and as a conclusion "Takeda (Physiol. Instit., Marburg), Pfluger's Arch., 1Z9, 82, 1909.
«0. Folin, C. C. Erdmann (Waverley), Jour, of Biol. Chem., 3, 83, 1907; 8,41,57, 1910; 9, 85, 1911.
*T. Kinoshita (University Physiol. Instit., Vienna), Centralbl.


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